2 edition of Application of image analysis to fungal fermentations found in the catalog.
Application of image analysis to fungal fermentations
Philip William Cox
Thesis (Ph.D) - University of Birmingham, School of Chemical Engineering, Faculty of Engineering, 2000.
|Statement||by Philip William Cox.|
|The Physical Object|
|Number of Pages||270|
Abstract Abstract Background Although the citric acid fermentation by Aspergillus niger is one of the most important industrial microbial processes and various aspects of the fermentation appear in a very large number of publications since the s, the effect of the spore inoculum level on fungal morphology is a rather neglected : Mattey Michael and Papagianni Maria. IntechOpen is a leading global publisher of Journals and Books within the fields of Science, Technology and Medicine. We are the preferred choice of o authors worldwide.
Master the art and science of specimen collection, preparation, and evaluation with Canine & Feline Cytology: A Color Atlas and Interpretation Guide, Second Edition. This easy-to-use guide covers all body systems and fluids including a special chapter on acquisition and management of cytology specimens. Hundreds of vivid color images of normal tissue alongside abnormal tissue images . Batch fermentations were carried out in a stirred tank bioreactor, which were inoculated directly with spores in concentrations ranging from 10 4 to 10 9 spores per ml. Morphological features, evaluated by digital image analysis, were classified using an artificial neural network (ANN), which considered four main object types: globular and elongated Cited by:
the image and enhanced, often to produce a 'binary image' w h ere a p ix el is either bright (o n) or dark (o ti). Such images where the 'on' pixels are given false colours are shown in Figs 1b, 2b and 3b. Appropriate analysis then provides the quantitative information required. The main advantage ofimage analysis is that repeti. Fungal cell morphology is an important factor that affects fumaric acid production via fermentation. In the present study, pellet and dispersed mycelia morphology of Rhizopus arrhizus NRRL was analysed using image analysis software and the impact on fumaric acid production was evaluated. Batch experiments were carried out in shake flasks.
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A semiautomatic image analysis protocol that was developed to characterize the mycelium morphology during the fermentation process based on four morphological types (unbranched, branched, entangled, and clumped microorganisms) was applied to study the effect of agitation on the morphology of T.
reesei. It was shown via statistical validation that broth samples used for image analysis represented the whole population of the fungi Cited by: Abstract.
Image analysis is now a well established complement to optical microscopy, allowing routine quantification of microscopic observations. Recent method developments include location and enumeration of bacteria in solid foods, in situ microscopy and image analysis for on-line monitoring of yeast fermentations, Cited by: Application of image analysis to fungal fermentations.
Author: Cox, Philip William. ISNI: Awarding Body: University of Birmingham Current Institution: University of Birmingham Date of Award: Availability of Full Text. In order to better understand the relationship between viscosity changes and fungus morphology during fermentations of Trichoderma reesei, an accurate image analysis method has been developed to.
Application of image analysis to fungal fermentations. (Thesis) Cox PW. Publisher: University of Birmingham  Metadata Source: The British Library Type: Thesis.
Abstract. Highlight Terms No biological terms identified No abstract supplied. Menu Formats. Abstract; Thesis at EThOS. Fully-automatic image analysis methods are described for the measurement of vacuolation in filamentous fungi growing in dispersed form in submerged cultures.
For Penicillium chrysogenum biomass from to 38 gL -L, volumes of cytoplasmic and vacuolised or degenerated regions can be converted successfully into dry weight estimations, even when Author: C.R.
Thomas, G.C. Paul, C.A. Kent, H.L. Packer, E. Keshavarz-moore, M.D. Lilly. Abstract. Fungal spores are used in the laboratory for culture maintenance and at laboratory and other scales as inocula for fermentations.
The spore swelling and germination processes constitute a major part of the lag phase, and the subsequent culture morphology and productivity can be greatly influenced by the initial concentration.
1 Review. The pace of progress in fermentation microbiology and biotechnology is fast and furious, with new applications being implemented that are resulting in a spectrum of new products, from renewable energy to solvents and pharmaceuticals.2/5(1). In most cases, the carbon source is the same compound used to provide energy.
Carbohydrates are generally preferred as carbon and energy sources; however, many yeasts can also grow on other organic compounds, including lower aliphatic alcohols, sugar alco- hols, organic acids, fatty acids, amino acids. The aim of this book is to cover the basics of image analysis from a statistical per-spective, and with emphasis on methods relevant to the biological sciences.
The book is written for both biological scientists and applied statisticians, whom, we assume, are mainly interested in analysing images of static objects in laboratory-type situations. An image-analysis scheme to detect fungal infections in digital fluorescence microscopy images is presented.
The scheme consists of the image preprocessing, segmentation, parameterization, and classification steps that all have a strong influence on the detection by: 2.
Digital image analysis is a powerful tool for getting relevant information in order to establish the mechanisms of mass transfer as well as to evaluate the viability of the mycelia. This review focuses on (a) the main characteristics of the two most common morphologies exhibited by filamentous microorganisms;Cited by: tiation in solid state culture via a novel image analysis method," in Abstracts of European BioPerspectives, K oln, Germany, May 3.
Barry, C. Chan, and G. Williams, \Morphological quanti cation of la-mentous fungal development using membrane immobilization and automatic image analysis," J Ind Microbiol Biot, vol.
36, no. 6, pp. Digital image analysis is the state of the art method to characterize and quantify fungal morphology in the differentiation process from spores to filamentous structures to by: This review is focused on using computer image analysis as a means of objective and quantitative characterizing optical images of the macroscopic (e.g.
microbial colo- nies) and the microscopic (e.g. single cell) objects in the microbiological Size: KB. A link between vacuolation and fragmentation of Penicillium chrysogenum mycelia in stirred tank submerged fermentations is reported. Quantitative information on vocuolation and morphology was obtained by image analysis.
In fed‐batch fermentations the coincidence of the events of rapid vacuolation and the fall of the mean total and main hyphal lengths suggests that hyphal. Fermentation Basics Fermentation is the term used by microbiologists to describe any process for the production of a product by means of the mass culture of a microorganism.
The product can either be: 1. The cell itself: referred to as biomass Size: 87KB. Purchase Analytical Biotechnology - 1st Edition. Print Book & E-Book. ISBNBook Edition: 1. Fermentation of food arose more by accident than by guided efforts.
Fermentation has been used since ancient times to conserve and alter foods. For thousand of years fermentation processes were carried out without understanding microbial mechanisms, until XIX century. Fruit fermentation is a natural process and in this contextFile Size: KB.
Filamentous fungi are widely used in the biotechnology industry for the production of industrial enzymes. Thus, considerable work has been done with the purpose of characterizing these by:. Chapter 2 Application of image analysis in the fungal fermentation of Trichoderma reew?/RUT-C30 8 Abstract 9 Introduction 9 Materials and Methods 11 Strain 11 Culture 11 Control Strategies 12 Bioreactors 12 Image Analysis 13 Results and discussions 13 Data validation 13 Morphological Parameters 22 Conclusion 30 Acknowledgment 31 References Abstract.
Although the citric acid fermentation by Aspergillus niger is one of the most important industrial microbial processes and various aspects of the fermentation appear inCited by: Fungal Diversity 45 Induction of antibiotic production of freshwater fungi using mix-culture fermentation Wai Hong Ho*, Pui Chi To and Kevin D.
Hyde Centre for Research in Fungal Diversity, Department of Ecology & Biodiversity, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, PR China Ho, W.H., To, P.C. and Hyde, K.D. ().Cited by: